Research Article
Open Access
Curcumin Complex-DNA Interaction Studied By Fourier Transform Infrared Spectroscopy
K.Senthil1, P.M.Andavan2
1Research Scholar, Bharathiar University, Cbe, India
2Department of Physics, Government Arts College, Cbe, India
2Department of Physics, Government Arts College, Cbe, India
K.Senthil et al /Int.J. TechnoChem Res. 2016,2(3),pp 205-211.
Abstract
Curcumin is a non toxic, a dietary natural polyphenol known for its potent anti-inflammatory,
antioxidant, antimutagenic and anticarcinogenic effects. The interaction of curcumin Complex(Curcumin
and metal ions) with DNA is widely used in clinical treatment of a variety of neoplastic diseases, but its
therapeutic effects is not fully understood. We here report the binding mechanism and structural
properties of curcumin with DNA considered as a therapeutic target. The interaction of DNA with
curcumin is investigated in aqueous solution at physiological pH with durg/DNA molar ratios of r=1:40,
1:20, 1:10, 1:5 and 1:2. Fourier Transform Infrared(FT-IR) and UV-absorption spectroscopy are used to
determine the secondary structure of drug and DNA, drug binding sites, binding constant, as well as the
structural variations of curcumin-DNA complexes in aqueous solution. Our spectroscopic result showed
that interaction occurred mainly through G-C ,A-T base pairs and major interaction with phosphate
groups with overall binding constant of K= 2.95×105
M-1. Curcumin complexes maybe induced
biopolymer conformational changes with DNA in the presence of metal ions.
antioxidant, antimutagenic and anticarcinogenic effects. The interaction of curcumin Complex(Curcumin
and metal ions) with DNA is widely used in clinical treatment of a variety of neoplastic diseases, but its
therapeutic effects is not fully understood. We here report the binding mechanism and structural
properties of curcumin with DNA considered as a therapeutic target. The interaction of DNA with
curcumin is investigated in aqueous solution at physiological pH with durg/DNA molar ratios of r=1:40,
1:20, 1:10, 1:5 and 1:2. Fourier Transform Infrared(FT-IR) and UV-absorption spectroscopy are used to
determine the secondary structure of drug and DNA, drug binding sites, binding constant, as well as the
structural variations of curcumin-DNA complexes in aqueous solution. Our spectroscopic result showed
that interaction occurred mainly through G-C ,A-T base pairs and major interaction with phosphate
groups with overall binding constant of K= 2.95×105
M-1. Curcumin complexes maybe induced
biopolymer conformational changes with DNA in the presence of metal ions.
Keywords
DNA, Curcumin; FT-IR spectroscopy.
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