Review Article
Open Access
Designing a Glycoprotein B-Based Vaccine Candidate Against Infectious Laryngotracheitis Virus Through Immunoinformatics Approaches
Ghoneim Elmehrath*, Ortega Tirado
Bioscience Laboratory, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil
Elmehrath G, Tirado O. Human Amniotic Membrane Scaffold Use in Wound Care: Macrophage Activation and Healing Outcomes, Accounts of Biotechnology Research. 2023, Vol. 11 No. 2: 105
Abstract
Microalgae biomass recovery and vaccine development are pivotal areas in
biotechnology, requiring innovative and efficient strategies. This review focuses
on chemical-based flocculation methods for Chlorella sorokiniana biomass
recovery and immunoinformatics-based design of glycoprotein B (gB) vaccines
against Infectious Laryngotracheitis Virus (ILTV). Various flocculating agents,
including ferric chloride, aluminum sulfate, zinc sulfate, and sodium hydroxide,
were assessed for their recovery efficiency. Among these, ferric chloride
demonstrated superior performance, achieving nearly 80% biomass recovery in
10 minutes at 0.75 g/L concentration. Zinc sulfate showed the least efficacy. In
parallel, B-cell epitope prediction for ILTV gB identified the PH-1 region as highly
antigenic, hydrophilic, and structurally stable, making it a promising candidate for
subunit vaccine development. This review emphasizes the integration of chemical
flocculation and immunoinformatics to improve bioproduct recovery and vaccine
design, offering cost-effective, environmentally friendly, and efficient solutions.
biotechnology, requiring innovative and efficient strategies. This review focuses
on chemical-based flocculation methods for Chlorella sorokiniana biomass
recovery and immunoinformatics-based design of glycoprotein B (gB) vaccines
against Infectious Laryngotracheitis Virus (ILTV). Various flocculating agents,
including ferric chloride, aluminum sulfate, zinc sulfate, and sodium hydroxide,
were assessed for their recovery efficiency. Among these, ferric chloride
demonstrated superior performance, achieving nearly 80% biomass recovery in
10 minutes at 0.75 g/L concentration. Zinc sulfate showed the least efficacy. In
parallel, B-cell epitope prediction for ILTV gB identified the PH-1 region as highly
antigenic, hydrophilic, and structurally stable, making it a promising candidate for
subunit vaccine development. This review emphasizes the integration of chemical
flocculation and immunoinformatics to improve bioproduct recovery and vaccine
design, offering cost-effective, environmentally friendly, and efficient solutions.
Keywords
Flocculation; Sedimentation; Ferric Chloride; Glycoprotein B; Immunoinformatics; Vaccine Design.
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